Application

Alkaline Phosphatase Diethanolamine Activity Kit has been used:in the detection of alkaline phosphatase (ALP) levels in the blood serum and osteoblastsfor monitoring ALP levels during osteoblast differentiation in mouse myoblast C2C12 cellsfor determining ALP activity in the bone metastasis model of lung cancerfor measuring ALP levels in colon tissue homogenates

General description

The Alkaline Phosphatase Diethanolamine Detection Kit provides ready-to-use reagents for detecting the presence of alkaline phosphatase activity. This simple assay to detect alkaline phosphatase activity uses p-Nitrophenyl Phosphate (pNPP) as the substrate.

Principle

Alkaline phosphatase hydrolyzes pNPP to p-nitrophenol and inorganic phosphate. During incubation of the alkaline phosphatase sample and substrate at 37 C, the reaction is followed by monitoring the increase in absorbance at 405 nm.

Suitability

Suitable for the measurement of alkaline phosphatase activity in a variety of samples